Spectroscopy of Biopolymers           Syllabus for 2006 autumn semester

Web:      http://www.cyut.edu.tw/~wjchien        Syllabus Download 

 

Instructor:      Wei-Jyun Chien (錢偉鈞)                               Applied Chemistry, CYUT

Tel: 23323000 ext. 7230 or 4307,                   e-mail: wjchien@mail.cyut.edu.tw

Lecture:   Monday 13:30~14:20; Wednesday 10:10~12:00 (CYUT)

                Friday 14:00~17:00 (THU)

Textbooks:

1.        Physical Chemistry 1st ed.; Thomas Engel and Philip Reid; Pearson Education, Inc.

2.        Instrumental Analysis 4th ed.; Skoog and Leary; Fort Worth :/Saunders College Pub.

3.        Principles of Fluorescence Spectroscopy 2nd ed. J. R. Lakowicz, Kluwer Academic / Plenum Publisher. 1999

4.        Circular dichroism : principles and applications 2nd ed. edited by Nina Berova, Koji Nakanishi, Robert W. Woody.  Wiley-VCH , c2000

5.        NMR of Proteins and Nucleic Acids Kurt Wüthrich, Wiley International Publication. 1986.

6.        Infrared and Raman Spectroscopy, Methods and Applications, edited by Bernhard Schrader, VCH, 1995.

7.       Infrared Spectral Interoretation, A Systematic Approach Brian Smith, CRC Press, 1999.

e-books:

1.      Protein Structure and Function  by Gregory Petsko and Dagmar Ringe, New Science Press Ltd, 2004. http://www.new-science-press.com/browse/protein

2.       Protein Structure Determination in Solution by Two-Dimensional and Three-Dimensional Nuclear Magnetic Resonance Spectroscopy Angela M. Gronenborn* and G. Marius Clore* 2Anal. Chem. 1990, 62, 2-15.    http://pubs.acs.org/cgi-bin/archive.cgi/ancham/1990/62/i01/pdf/ac00200a003.pdf  

Schedule:

Contents

Slides

Text

Reading Assignments

10

11

12

13

14

15

16

Introduction – Resources and tools

S1-1

 

 

17

18

19

20

21

22

23

Introduction to Biopolymers I - peptides and proteins

S2-1,S2-2

 

 

24

25

26

27

28

29

30

Introduction to Biopolymers II – nucleic acids and hydrocarbons

S2-3, S2-4

 

R1, R2, R3, R4

10/1

2

3

4

5

6

7

Introduction to Spectroscopy I -Vibrational & Rotational Spectroscopy

S3

T3

 

8

9

10

11

12

13

14

Applications of IR Spectroscopy on Biopolymers I

S3-1

 

R1, R2, R3

15

16

17

18

19

20

21

Applications of IR Spectroscopy on Biopolymers II

S3-2

 

 

22

23

24

25

26

27

28

Introduction to Spectroscopy II - Electronic Spectroscopy

S4-1, S4-2

T4

 

29

30

31

11/1

2

3

4

Applications of UV-Vis Spectroscopy

 

 

 

5

6

7

8

9

10

11

Applications of Fluorescence Spectroscopy on Biopolymers I

 

 

 

12

13

14

15

16

17

18

Applications of Fluorescence Spectroscopy on Biopolymers II

 

 

 

19

20

21

22

23

24

25

Introduction to Spectroscopy III – Circular Dichroism Spectroscopy

S5-1

 

 

26

27

28

29

30

12/1

2

Applications of CD Spectroscopy on Biopolymers I

S5-2

 

 

3

4

5

6

7

8

9

Applications of CD Spectroscopy on Biopolymers II

S5-3

 

 

10

11

12

13

14

15

16

Introduction to Spectroscopy IV – NMR Spectroscopy

 S6-1

 

 R1, R2, R3, R4

17

18

19

20

21

22

23

NMR Spectroscopy and Protein Conformational Study

 S6-2

 

 

24

25

26

27

28

29

30

NMR Spectroscopy and Protein Conformational Study

 S6-3

 

 

31

1/1

2

3

4

5

6

Application of NMR techniques on Biopolymers

 

 

 

7

8

9

10

11

12

13

Review

 

 

 

14

15

16

17

18

19

20

Final Exam